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Dimerized PD-L1 by BMS-202 treatment was internalized into early endosomes and delivered to lysosomes. HeLaM cells co-expressing GFP-PD-L1 and mCherry-Rab5 or mCherry-Lgp120 were treated with BMS-202. ( A ) GFP-PD-L1, which was localized to the cell surface, Rab10-positive tubules, and Rab10-negative small vesicles in HeLaM cells at 0 min, was internalized into Rab5-positive early endosomes 30 min after BMS-202 treatment. ( B ) After120 min, GFP-PD-L1 accumulated in perinuclear vesicles. Some of these are mCherry-Lgp120-positive lysosomes. Bars = 10 μm.

Journal: Acta Histochemica et Cytochemica

Article Title: Intracellular Localization of PD-L1 in Rab10-positive Open Tubular Endosome System of Cancer Cells

doi: 10.1267/ahc.25-00060

Figure Lengend Snippet: Dimerized PD-L1 by BMS-202 treatment was internalized into early endosomes and delivered to lysosomes. HeLaM cells co-expressing GFP-PD-L1 and mCherry-Rab5 or mCherry-Lgp120 were treated with BMS-202. ( A ) GFP-PD-L1, which was localized to the cell surface, Rab10-positive tubules, and Rab10-negative small vesicles in HeLaM cells at 0 min, was internalized into Rab5-positive early endosomes 30 min after BMS-202 treatment. ( B ) After120 min, GFP-PD-L1 accumulated in perinuclear vesicles. Some of these are mCherry-Lgp120-positive lysosomes. Bars = 10 μm.

Article Snippet: BMS-202 (PD-1/PD-L1 inhibitor 2) was purchased from Selleck Biotech (Yokohama, Japan).

Techniques: Expressing

Expression of mCherry-CMTM6 retains PD-L1 on the cell surface and tubules even after BMS-202 treatment. HeLaM cells co-expressing GFP-PD-L1 and mCherry-CMTM6 were treated with BMS-202. In cells overexpressing CMTM6, GFP-PD-L1 was predominantly observed on the membranes of cell surface and Rab10-positive tubules even after BMS-202 treatment. Bars = 10 μm.

Journal: Acta Histochemica et Cytochemica

Article Title: Intracellular Localization of PD-L1 in Rab10-positive Open Tubular Endosome System of Cancer Cells

doi: 10.1267/ahc.25-00060

Figure Lengend Snippet: Expression of mCherry-CMTM6 retains PD-L1 on the cell surface and tubules even after BMS-202 treatment. HeLaM cells co-expressing GFP-PD-L1 and mCherry-CMTM6 were treated with BMS-202. In cells overexpressing CMTM6, GFP-PD-L1 was predominantly observed on the membranes of cell surface and Rab10-positive tubules even after BMS-202 treatment. Bars = 10 μm.

Article Snippet: BMS-202 (PD-1/PD-L1 inhibitor 2) was purchased from Selleck Biotech (Yokohama, Japan).

Techniques: Expressing